ノーベル賞受賞者、山中伸也教授の論文
ノーベル賞受賞者、山中伸也教授の論文
ノーベル賞受賞者、山中伸也教授の論文が京都大学図書館サイトで公開されました。
「Cell」に２００６年に掲載されたものです。
Title
Induction of Pluripotent Stem Cellsfrom Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
Kazutoshi Takahashi 1 and Shinya Yamanaka 1,2,
1
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
2
CREST, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan
Contact: yamanaka@frontier.kyoto-u.ac.jp
DOI 10.1016/j.cell.2006.07.024
SUMMARY
Differentiated cells can be reprogrammed to anembryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by
introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions.
Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all
three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Cell 126, 663–676, August 25, 2006 ª2006 Elsevier Inc.
リンク先
http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/159777/1/j.cell.2006.07.024.pdf
